Explain your answers to the following. A. The binding affinity between a peptide agonist and its specific G protein coupled receptor involves which types of chemical bonds? B. The binding affinity between a peptide agonist and its specific G protein coupled receptor alone determines the sensitivity of a cell to the agonist peptide. 2. True or False. Explain your answe r. G beta gamma subunits initiate MAPK cascade signaling via SH domains. 3. D e scribe how you would experimentally demonstrate the requirement for a G protien in agonist-stimlulated PLC activity 4. Describe how you would experimentally demonstrate that a specific G protein subunit beta is required for peptide agonist-stimulated increases in MAPK activity. 5. Describe how the GPCR and MAPK pathways ‘cross-talk’ or interact with each other.
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